UI - 11776612
AU - Zheng S; Ke Y
[Study of APC, Rb, c-met gene copy numbers of human gastric mucosa
epithelial cell line GES-1]
SO - Zhonghua Zhong Liu Za Zhi 1999 Nov;21(6):409-11
AD - Beijing Institute for Cancer Research, School of Oncology, Beijing
Medical University, Beijing 100034.
OBJECTIVE: To study the copy number of oncogene/tumor suppressor genes
in GES-1 cell line to identify its characteristics. METHODS: Bio-14-dATP
was incorporated into APC, Rb, c-met gene cloned in plasmid by nick
translation. Fluorescence in situ hybridization (FISH) of interphase
nuclei of GES-1 cells was performed. RESULTS: Two copies of APC were
shown in 48% interphase nuclei and 3 copies in 22%; 71% and 80% cells
had normal copies of Rb and c-met genes in their nuclei, respectively.
CONCLUSION: GES-1 cell line is a relatively normal gastric mucosa
epithelial cell line and can be used as a human in vitro model system
for the study of carcinogenesis.
UI - 11807886
AU - Tharapel SA; Kadandale JS
Primed in situ labeling (PRINS) for evaluation of gene deletions in
SO - Am J Med Genet 2002 Jan 15;107(2):123-6
AD - Cytogenetics Reference Laboratory, Pathology and Laboratory Medicine
Service, V.A. Medical Center, Tennessee 38104, USA.
Rearrangements involving the 13q14 and 17p13 chromosomal regions are
often observed in leukemias and lymphomas. These rearrangements are not
always identifiable cytogenetically. In more than 50% of cases,
deletions occur at the submicroscopic level and the karyotypes appear
normal. Molecular cytogenetic techniques such as fluorescence in situ
hybridization (FISH) have accordingly contributed to the identification
of a variety of subtle rearrangements such as those involving
submicroscopic deletions. However, FISH is expensive, time consuming,
technically burdensome, and requires cloned DNA probes. A newer
technique, primed in situ labeling (PRINS), has been tested as a
possible alternative to FISH. To assess the utility and efficiency of
the PRINS method in the detection of RB1 and p53 deletions, we evaluated
10 patients with hematological disorders and known rearrangements, i.e.,
deletions involving 13q14 and 17p13 regions. The data in these cases
were validated against data obtained with standard FISH probes. Our
results indicate that PRINS could be used with relative ease in
cytogenetics laboratories and could serve as an alternative to
conventional FISH for defining deletions involving unique sequences.
Copyright 2001 Wiley-Liss, Inc.
UI - 11724429
AU - Ferri G; Colalongo C; Bini C; Pelotti S; Pappalardo G
DNA typing of hair shafts by microwave irradiation: real or deceptive
SO - Int J Legal Med 2001 Oct;115(2):118-20
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