Facile Detection of Mitochondrial DNA Mutations in Tumors and bodily Fluids
Makiko S. Fliss, Henning Usadel, Otavia L. Caballero, et al.
Abramson Cancer Center of the University of Pennsylvania
Last Modified: November 1, 2001
Reviewers: Li Liu, MD
Source: Science, Volume 287(5460): 2017-2019, (March) 2000
Précis: Bodily fluid testing may detect come cancers
IntroductionAny non-invasive techniques that would aid early detection of cancers would clearly be welcomed. Some studies have demonstrated that high levels of mitochondrial DNA (mtDNA) are ubiquitous in human cells and most copies of mtDNA are identical at birth. Even slight alterations in DNA sequences could have profound effects, including cancer formation. In this study, the researchers evaluated the mtDNA in tumor samples and bodily fluids of cancer patients.
MethodA total of 14 bladder, 13 head and neck, and 14 lung cancer samples were evaluated for mtDNA mutations and the results were compared with mtDNA from patients? bodily fluids.
- 292 mtDNA sequence variations were detected in cancer samples.
- Mutant mtDNA derived from tumor tissues was found in all urine samples obtained from patients with bladder cancer, in two third of the saliva samples from patients with head and neck cancer, and in 80% bronchoalveolar lavage samples from patients with lung cancer.