The Pap Test: Cervical Changes and Further Testing

Modified by: Lara Bonner Millar, MD
By: Ivor Benjamin, MD and Linda Echols, MSN, RN
Abramson Cancer Center of the University of Pennsylvania
Last Modified: March 12, 2012

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You have been informed that your recent Pap smear showed some changes in the cells of your cervix and you need further testing. This document will help you understand the cervical cell changes and the probable course of management.

What is a Pap Test?

The Pap test, also called a "Pap smear" was introduced as a screening test for cervical cancer in 1943 by Dr. George Papanicolaou, for whom the test is named. The Pap test examines cells from the cervix, which is located at the top of the vagina. The American College of Gynecology recommends testing at age 21 and if normal, then every 2 years thereafter. However, those age 30 and over who have had three consecutive negative (i.e., normal) test results may be screened once every 3 years.

Normal vaginal discharge contains cells that are shed from the cervix and uterus. Samples of these cells are taken for the Pap test. For this reason, you should not douche, have vaginal intercourse, use tampons or vaginal medication for 48 hours before the Pap test is done.

During the gynecologic examination, a speculum is inserted into the vagina so that the cervix and vagina can be seen. The doctor or nurse inserts a cotton-tipped swab or gentle cytobrush into the cervical opening (cervical os) to sample endocervical cells (which come from the area most at risk for cervical cancer). Next, the examiner gently scrapes the outside of the cervix (portio) with a small spatula in order to get samples of the discharge containing cervical cells.

These samples are sent to a pathologist for detailed examination under a microscope. A report is sent to your doctor with a classification of the test results and a description of the cell changes. An HPV test, looking for certain strains of the HPV virus associated with cervical cancer, can also be sent with this sample. This is done by obtaining cervical cells in the same manner as is done for a Pap smear. This test is often routinely done at the time of a Pap smear, or alternatively as a follow-up test after an abnormal Pap smear.

Process of Cervical Changes

The cervix is the part of the uterus that extends into the vagina. There are two types of cells that line the cervix, one lines the outer cervix (portio) and another lines the inner cervix (endocervix). There is a distinct junction between the two cell types called the transformation zone. The Pap test is taken from this area because this where dysplasia (pre-cancer) and cancer most often arise.

Two common changes in cells are metaplasia and dysplasia.

Metaplasia - Metaplasia is generally described as a process of cell growth or cell repair that is benign (not cancerous). This process normally occurs in unborn babies, during adolescence, and with the first pregnancy. Studies have shown that metaplasia is present in more than one half of all women at some point in their development. This is a normal finding and does not indicate cancer.

Dysplasia - In dysplasia, there is an increase in the number of cells formed, which do not mature as expected. This changes the inside of the cell. The higher the grade of dysplasia found on the cervix, the more likely that it will progress to invasive cancer. For this reason, dysplasia is thought of as a "pre-cancerous" condition. Dysplasias are nearly 100% curable if managed appropriately. Although some mild dysplasias (LSIL) will regress without treatment, it is not possible distinguish between dysplastic areas of the cervix that will return to normal and dysplastic areas which will progress and ultimately become cancer. In turn, these results necessitate further testing.

Causes of Cervical Cell Changes

Inflammation often results in mildly abnormal Pap tests resulting in the diagnosis of ASCUS in the Bethesda System or changes consistent with Human Papilloma Virus (HPV) infection. An inflamed cervix may appear red, irritated, or eroded. Some of the common causes of cervical inflammation are:

  • Bacteria (from an infection)
  • Viruses, especially herpes infections and condyloma cuminata (warts)
  • Yeast or monilia infections
  • Trichomonas infections
  • Pregnancy, miscarriage, or abortion
  • Chemicals (for example, medications)
  • Hormonal changes

When the inflammation is treated and cleared, repair through metaplasia usually will follow. In several months, a repeat Pap test will often then be normal.

Classification of Squamous Cells on the Pap Test

Several different classification schemes have evolved over the years for characterizing Pap test results. Unfortunately, this is a continuing source of confusion. The most commonly used classification scheme is the Bethesda System.

  • ASC - atypical squamous cells. This is the most common abnormal finding in Pap tests. In fact, of theover 50 million abnormal Pap tests each year, only 3.5 million are abnormal, and only about ten thousand women are diagnosed each year with invasive cervical cancer.  The Bethesda System divides this category into two groups:
    • ASC-US - atypical squamous cells of undetermined significance. The squamous cells do not appear completely normal, but doctors are uncertain about what the cell changes mean. Sometimes the changes are related to HPV infection, but they can also be caused by other factors such as pregnancy. For women who have ASC-US, her cells may then be tested for the presence of high-risk HPV. If HPV is present, colposcopy (see below) will usually be done. Alternatively, the pap test may be repeated in 6 months, and if normal, she may resume the usual schedule of screening. If a woman has ASC-US on her pap with a negative test for high-risk subtypes of HPV, a pap and HPV test may just be repeated in one year without further testing.
    • ASC-H - atypical squamous cells, cannot exclude a high-grade squamous intraepithelial lesion. Intraepithelial refers to cells on the surface of the cervix. ASC-H cells do not appear normal, but doctors are uncertain about what the cell changes mean. However, there is a possibility that a pre-cancerous lesion is developing, so a colposcopy is recommended.
  • LSIL - low-grade squamous intraepithelial lesion; this is the earliest pre-cancerous lesion. LSILs may be referred to as mild dysplasia or as cervical intraepithelial neoplasia type 1 (CIN-1). Although many LSIL lesions will resolve on their own, there is no way to predict which ones will resolve, so doctors will typically perform a colposcopy.
  • HSIL - high-grade squamous intraepithelial lesion. HSILs are more abnormal looking than LSILs and have a higher likelihood of progressing to cancer. HSILs include lesions that in other classification systems may be referred to as moderate or severe dysplasia, carcinoma in situ, and/or CIN-2 and CIN-3. A finding of HSIL necessitates a colposcopy.
  • Squamous cell carcinoma is the most advanced category. This means that abnormal cervical squamous cells have invaded into the cervix. A finding of squamous cell carcinoma requires further testing and treatment. Keep in mind, when women undergo appropriate screening, most of the time, abnormalities in the cervix are detected and treated before they have had the chance to progress to cervical cancer.

Classification of Glandular Cells on the Pap Test

Glandular cells, which produce mucus, are found in the opening of the cervix and in the uterus. Abnormalities in these cells are more difficult to classify. Glandular cells that are seen on the Pap test most commonly come from the endocervix (area closest to the uterus). However, other glandular epithelial surfaces in the female reproductive tract may shed cells that are visible on the Pap test. Endometrial cells may also appear on Pap tests and reveal underlying abnormalities. Because the female reproductive tract is open to the abdominal cavity via the Fallopian tubes, occasionally, cells from the ovary, Fallopian tubes, peritoneum or other interabdominal organs may be seen on the Pap smear. Glandular cells on the the Pap test are classified as follows:

  • Endometrial cells, cytologically benign, in a postmenopausal woman
  • Atypical glandular cells (AGC, formerly AGUS) that should be qualified further, if possible, as to whether a reactive or neoplastic process is favored. A finding of AGC will typically be followed by a colposcopy and possibly also endometrial sampling.
  • Endocervical Adenocarcinoma
  • Endometrial Adenocarcinoma
  • Extrauterine Adenocarcinoma (e.g. ovarian, Fallopian tube, pancreas, etc.)
  • Adenocarcinoma, not otherwise specified (i.e. unknown primary site)

Colposcopy: The next diagnostic step

All abnormal Pap smear result often requires further evaluation. If the abnormality is minor (i.e. inflammation, or HPV changes) your healthcare provider may choose to repeat the Pap test in a few months, as your own immune system may "clear" the HPV infection and a follow up Pap be normal. If the abnormalities have persisted or worsened, colposcopy is indicated. Colposcopy will enable your physician or nurse to make a more accurate diagnosis.

Colposcopy - A colposcope is a lighted microscope that is used to magnify the cervical tissue during a pelvic examination. The colposcope is used to visualize abnormal areas of the cervix and vagina that are too small to see with the naked eye. The entire transformation zone must be seen. The colposcopic examination is an office procedure and may be a bitmore uncomfortable than a routine pelvic examination because of the pressure from the speculum lasting longer than a typical Pap test. The test takes 5 to 10 minutes to perform. During the examination, the examiner may take small samples of cervical tissue (biopsies), which are later examined by a pathologist. These diagnostic biopsies will guide further management.

Colposcope from examiner's view
From the examiner's perspective

Colposcope from the patient's view
From the patient's perspective

Treatment Options for Cervical Dysplasia

Cone Biopsy - A cone biopsy is a minor operation, which is usually performed in an outpatient surgical facility. In the operating room, the physician removes a small cone shaped tissue sample from your inner cervix. This tissue is sent to a pathologist for detailed examination under a microscope. This procedure does not remove any of your reproductive organs and should have little impact on your future ability to become pregnant. If only dysplasia is found in the cone specimen, then often no additional treatment will be required. However, if invasive cancer is discovered, additional treatment (i.e. surgery or radiation therapy) is indicated. Therefore, a cone biopsy may be considered as therapeutic (if all of the dysplasia is removed) or diagnostic (if it discovers a worse problem that requires additional treatment).

Loop Electrosurgical Excision Procedure (LEEP) - The LEEP procedure is similar to a cone biopsy in that it removes a tissue sample from your cervix, which is then examined, under a microscope, by a pathologist. It may also be called a LLETZ (large loop excision of the transformational zone). The LEEP procedure uses a low voltage, electric wire to cut away the abnormal area and has the advantage of being easily performed in the office with local anesthesia. However, the LEEP procedure and cone biopsy are not equivalent and your physician will recommend which is the best option, depending on your case.

Cryosurgery - Cryosurgery is another treatment option that can be performed in the doctor's office. During the procedure, the doctor freezes and thereby destroys the dysplasia on your cervix. You may notice a brief unpleasant cold sensation during the freezing procedure. A disadvantage of cryosurgery is that no specimen is obtained for the pathologist to examine in order to exclude the possibility of invasive cancer.

References & Further Reading

American Congress of Obstetricians and Gynecologists: Understanding Abnormal Pap Test Results and Human Papillomavirus Infection.

Centers for Disease Control "Cervical Cancer Screening"

Wright T, Massad L, Dunton C, et al. 2006 Consensus Guidelines for the Management of Women with Abnormal Cervical Cancer Screening Tests. American Journal of Obstetrics and Gynecology (2007;197(4):346-355).



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